Reactive changes in the adolescent porcine spine with disc degeneration due to endplate injury

S. Holm; A. Baranto; A. Kaigle Holm; L. Ekström; L. Swärd; T. Hansson; H.-A. Hansson

doi:10.1055/s-0037-1616581

Veterinary and Comparative Orthopaedics and Traumatology, Table of Contents

Vet Comp Orthop Traumatol 2007; 20(01): 12-17
DOI: 10.1055/s-0037-1616581

Original Research

Schattauer GmbH

Reactive changes in the adolescent porcine spine with disc degeneration due to endplate injury

Authors

S. Holm

¹Department of Orthopaedics, Sahlgrenska University Hospital, and Institute of Anatomy and Cell Biology, Göteborg University, Göteborg, Sweden
A. Baranto

¹Department of Orthopaedics, Sahlgrenska University Hospital, and Institute of Anatomy and Cell Biology, Göteborg University, Göteborg, Sweden
A. Kaigle Holm

¹Department of Orthopaedics, Sahlgrenska University Hospital, and Institute of Anatomy and Cell Biology, Göteborg University, Göteborg, Sweden
L. Ekström

¹Department of Orthopaedics, Sahlgrenska University Hospital, and Institute of Anatomy and Cell Biology, Göteborg University, Göteborg, Sweden
L. Swärd

¹Department of Orthopaedics, Sahlgrenska University Hospital, and Institute of Anatomy and Cell Biology, Göteborg University, Göteborg, Sweden
T. Hansson

¹Department of Orthopaedics, Sahlgrenska University Hospital, and Institute of Anatomy and Cell Biology, Göteborg University, Göteborg, Sweden
H.-A. Hansson

¹Department of Orthopaedics, Sahlgrenska University Hospital, and Institute of Anatomy and Cell Biology, Göteborg University, Göteborg, Sweden

Abstract

Summary

Degenerative and reactive structural alterations occurring after experimentally-induced disc degeneration were evaluated using a porcine model. A cranial perforation was made through the L4 vertebral endplate into the nucleus pulposus. Three months later, the lumbar intervertebral disc and adjacent vertebrae were dissected, fixed in formalin and further processed for histopathological analyses. The results showed that there were nucleus pulposus fragments, rather than a distinct border between the nucleus and annulus fibrosus. The central lamellae were distorted and delamination of the outer anterior layers was observed. Blood vessels emerged from the adjacent tissue, penetrated the annulus and branched into the residues of the nucleus. Nerve fibres accompanying the blood vessels could be recognized in the disc within the connective scar tissue. The epiphyseal cartilage plates in the vertebrae were hypertrophic in several areas and there was bone formation directed towards the centre of the vertebral body and the disc. Hypertrophic hyaline cartilage, newly formed bone and scar tissue filled the injury canal. A slight chronic inflammatory reaction was evident along vascular buds. The reactive changes dominated over the degenerated features in the operated disc. Physiological loading enhanced the infiltration of various tissue types characterizing immature cartilage formation. Prominent neovascularisation of the central parts of the disc is likely to be of key importance in turning the degenerative features of the remaining tissue into reactive healthy structures.

Keywords

Disc injury - osteogenesis - chondrogenesis - epiphyseal cartilage - fibrosis

Full Text

References

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